LOCUS       sequence-145702-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for insertion of attP site (C-terminal tag plasmid design),
            Puromycin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK41
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctggggtaac ctttgagttc tctcagttgg gggactagtg aagttcctat actttctaga
       61 gaataggaac ttcaaataaa catatgggcg cgccctgttg gaatatacta ttcaacctac
      121 aaaaataacg ttaaacaaca ctactttata tttgatatga atggccacac cttttatgcc
      181 ataaaacata ttgtaagaga ataccactct ttttattcct tctttccttc ttgtacgttt
      241 tttgctgtga gtaggtcgtg gtgctggtgt tgcagttgaa ataacttaaa atataaatca
      301 taaaactcaa acataaactt gactatttat ttatttatta agaaaggagg taccggtggc
      361 gcgccatgac cgagtacaag cctacagtgc gactggccac acgcgatgac gttcccagag
      421 ccgttcggac gttggctgcc gcgtttgcgg actatcccgc aactcgccat actgtggacc
      481 ctgatcgcca catcgagagg gtgactgagc ttcaggagct gttcctgacc agggtagggc
      541 tggacatagg caaggtctgg gtggccgacg atggagccgc tgttgcggtg tggactaccc
      601 cagagagcgt ggaagcaggg gctgtctttg ccgaaattgg gccaaggatg gccgaactgt
      661 ctgggtcccg tttggctgcc caacagcaga tggaaggact gcttgcccca catcgaccca
      721 aggagcctgc atggttcctc gctaccgtgg gtgtgagccc agatcacca
//
