LOCUS       sequence-145703-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for insertion of attP site (C-terminal tag plasmid design),
            Puromycin-Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK41
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    255..288
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(367..386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(368..386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(692..710)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatagtcaag tttatgtttg agttttatga tttatatttt aagttatttc aactgcaaca
       61 ccagcaccac gacctactca cagcaaaaaa cgtacaagaa ggaaagaagg aataaaaaga
      121 gtggtattct cttacaatat gttttatggc ataaaaggtg tggccattca tatcaaatat
      181 aaagtagtgt tgtttaacgt tatttttgta ggttgaatag tatattccaa cagggcgcgc
      241 ccatatgttt atttgaagtt cctattctct agaaagtata ggaacttcac tagtccccca
      301 actgagagaa ctcaaaggtt accccagttg gggctcgagc catccggaag atctggcggc
      361 cgctctccct atagtgagtc gtattacgcc ggatggatat ggtgttcagg cacaagtgtt
      421 aaagcagttg attttattca ctatgatgaa aaaaacaatg aatggaacct gctccaagtt
      481 aaaaatagag ataataccga aaactcatcg agtagtaaga ttagagataa tacaacaata
      541 aaaaaatggt ttagaactta ctcacagcgt gatgctacta attgggacaa ttttccagat
      601 gaagtatcat ctaagaattt aaatgaagaa gacttcagag cttttgttaa aaattatttg
      661 gcaaaaataa tataattcgg ctgcaggggc ggcctcgtga tacgcctatt tttataggtt
      721 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      781 ggaaccccta tttgtttatt t
//
