LOCUS       sequence-145705-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector for C-terminal eGFP tag on Drosophila melanogaster 
            Act5C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pIS1
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(547..568)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctgtgctatg ttgccctcga ctttgagcag gagatggcca ccgctgccag cagctcctcg
       61 ttggagaagt cctacgagct gcccgacgga caggtgatca ccatcggcaa cgagcgtttc
      121 cgctgccccg aggccctgtt ccagccctcg ttcttgggaa tggaggcttg cggcatccac
      181 gagaccacct acaactccat catgaagtgt gatgtggata tccgtaagga tctgtatgcc
      241 aacaccgtgc tgtccggtgg caccaccatg taccctggca tcgccgaccg tatgcagaag
      301 gagatcaccg ccctggcacc gtcgaccatg aagatcaaga tcattgcccc gccagagcgc
      361 aagtactctg tctggatcgg tggctccatc ctggcttcgc tgtccacctt ccagcagatg
      421 tggatctcca agcaggagta cgacgagtcc ggcccctcca ttgtgcaccg caagtgcttc
      481 ggatcttccg gatggctcga gatggtgagc aagggcgagg agctgttcac cggggtggtg
      541 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      601 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      661 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      721 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      781 gtccaggagc gcaccatctt cttcaaggac gacggcaact ac
//
