LOCUS       sequence-145706-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector for C-terminal eGFP tag on Drosophila melanogaster 
            Act5C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pIS1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     289..310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(362..395)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
       61 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      121 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      241 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaactag
      361 tgaagttcct atactttcta gagaatagga acttcaaata aacatatgct gttggaatat
      421 actattcaac ctacaaaaat aacgttaaac aacactactt tatatttgat atgaatggcc
      481 acacctttta tgccataaaa catattgtaa gagaatacca ctctttttat tccttctttc
      541 cttcttgtac gttttttgct gtgagtaggt cgtggtgctg gtgttgcagt tgaaataact
      601 taaaatataa atcataaaac tcaaacataa acttgactat ttatttattt attaagaaag
      661 gaggtaccgg tatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
      721 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
      781 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
//
