LOCUS       sequence-145720-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of cytosolic APEX2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pTRC-APEX2
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     196..213
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(280..1026)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct
       61 actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc
      121 ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga
      181 ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa
      241 cagccaagct tgcctgcagg tcgactctag cggatcttag gcatcagcaa acccaagctc
      301 ggaaagcttt tggtgagcct cagcgtaatc agcaaagaag gcatcttcgt ccgctgcata
      361 tttgtcaacg agagggcgga atacagggtc agacaaaaga gccttgtcag aaggtagctg
      421 aaggagacct tccttctcac cactcaacaa ctccgtgaag tatgagttgt cgaaaataag
      481 aggattagag gtccagggac cctcaaatcc agaacgctcc ttgtgtgcag ctccaatagt
      541 gtgaccccca gatagagcaa cgatatcttg gtcagtaagc cccatagctt tgccaaacac
      601 atctctcaaa tggtcagaac ccttagtggg atcgggcaag cgaccctctg gtggtggctc
      661 aggcttgtcc tctcttccag ggtggaatgg aaccttaggt ccacccgtga cctcaacggc
      721 aacaacgcca gccaactggt agaaatcggc gtagctcaaa atagggaact ccgccttgag
      781 tggctccaaa agcctaacag cgatgtcaag accgttgtta gcgctgtgag ccagttcggc
      841 agggtgcttg atggttccga agggtccacc ggtcttcgtg cccttgtcaa aggttccagc
      901 agagtggaat gccaaacgga gcattagagg agcgcatctc ttctcagcga tgaagcctct
      961 gagcttcttc ttcgccttct caacggcgtc ctggtaatca gcactcacag ttgggtaaga
     1021 ctttcc
//
