LOCUS       sequence-145736-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: , Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-BaxAlpha-C-15
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..734
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcgag ctgattaagg
       61 agaacatgcc catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
      121 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtcgtcg
      181 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
      241 gaaccttcat caagcaccct ccgggcatcc ccgacttctt taagcagtcc ttccctgagg
      301 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
      361 acaccagcct ccaggacggc tgcctcatct acaacgtcaa ggttagaggg gtgaacttcc
      421 cagccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagacga
      481 tgtaccccgc tgacggcggc ctggaaggcg catgtgacat ggccctgaag ctcgtgggcg
      541 ggggccacct gatctgcaac cttgagacca catacagatc caagaaaccc gctacgaacc
      601 tcaagatgcc cggcgtctac aacgtggacc acagactgga aagaatcaag gaggccgacg
      661 atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactctact ggtggcgctg
      721 gtgatggagg taaaggtgga ggaggttccg gactcagatc tcgagctcaa gcttcgaatt
      781 ctgcagtcga catggacggg tccggggaag c
//
