LOCUS       sequence-145739-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector encoding 3 Flag tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: 3XFlag-pLPC
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..171
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     LTR             403..872
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat ccatggacta caaagaccat
      121 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gcttgcggcc
      181 gcgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccga
      241 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
      301 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
      361 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
      421 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
      481 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
      541 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
      601 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
      661 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
      721 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
      781 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
      841 gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg
      901 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
      961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgaactcgct
     1021 gc
//
