LOCUS       sequence-145741-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector encoding 3 Flag tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: 3XFlag-pLPC
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..127)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(161..180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(162..180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(225..428)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(254..274)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        429..808
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctagatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc gcggccgcaa
       61 gcttgtcatc gtcatccttg taatcgatgt catgatcttt ataatcaccg tcatggtctt
      121 tgtagtccat ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta
      181 atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag
      241 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
      301 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
      361 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
      421 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
      481 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
      541 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
      601 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
      661 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
      721 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
      781 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
      841 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
      901 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcaggggag
      961 ca
//
