LOCUS       sequence-145745-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""All-in-one"" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA 
            with blue/white screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Findlay SD, Vincent KM, Berman JR, Postovit LM
  TITLE     A Digital PCR-Based Method for Efficient and Highly Specific 
            Screening of Genome Edited Cells.
  JOURNAL   PLoS One. 2016 Apr 18;11(4):e0153901. doi: 
            10.1371/journal.pone.0153901. eCollection 2016.
  PUBMED    27089539
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-18"; volume: "11(4):e0153901. doi"; pages: " 
            10.1371/journal.pone.0153901. eCollection 2016"
COMMENT     Sequence Label: All_in_one_CRISPR/Cas9_LacZ
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccaccatg gacaagaagt acagcatcgg cctggacatc
      121 ggtaccaaca gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaag
      181 ttcaaggtgc tgggcaacac cgaccgccac agcatcaaga agaacctgat cggcgccctg
      241 ctgttcgaca gcggcgagac cgccgaggcc acccgcctga agcgcaccgc ccgccgccgc
      301 tacacccgcc gcaagaaccg catctgctac ctgcaggaga tcttcagcaa cgagatggcc
      361 aaggtggacg acagcttctt ccaccgcctg gaggagagct tcctggtgga ggaggacaag
      421 aagcacgagc gccaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag
      481 taccccacca tctaccacct gcgcaagaag ctggtggaca gcaccgacaa ggccgacctg
      541 cgcctgatct acctggccct ggcccacatg atcaagttcc gcggccactt cctgatcgag
      601 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc
      661 tacaaccagc tgttcgagga gaaccccatc aacgccagcg gcgtggacgc caaggccatc
      721 ctgagcgccc gcctgagcaa gagccgccgc ctggagaacc tgatcgccca gctgcccggc
      781 gagaagaaga acggcctgtt cggcaacctg atcgccctga gcctgggcct gacccccaac
      841 ttcaagagca acttcgacct ggccgaggac gccaagctgc agctgagcaa ggacacctac
      901 gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga cctgttcctg
      961 gccgccaaga acctgagcga cgccatcctg ctgagcgaca tcctgcgcgt gaacaccgag
     1021 atcaccaagg cccccctgag cgccagcatg atcaagcgct acgacgagc
//
