LOCUS       sequence-145751-         460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mammalian expression of HCMV protein UL136. Expresses 
            only the 19kda isoform of UL136. Also expresses EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG1_UL136_19kDa_EE
FEATURES             Location/Qualifiers
     source          1..460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             424..441
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagattcta gagctagcga attcatggat gtgcccgacc cggaactcgg cgacccggcc
      121 cgccggccgt tgaacggggc catgtactac ggcagcggct gtcgcttcga cacggtggaa
      181 atggtggacg agacgagacc cgcgccgccg gcgctgtcat cgcccgaaac cggcgacgat
      241 agcaacgacg acgcggttgc cggcggaggt gctggcgggg taacatcatc cgcgactcgt
      301 acgacgtcgt cgaacgcgct gctgccagaa tggatggatg cggtacatgt ggcggtccaa
      361 gccgccgttc aagcgaccgt gcaagtaagt ggcccgcggg agaacgccgt atctcccgct
      421 acggagtaca tgcctatgga gtaaggatcc aattccgccc
//
