LOCUS       sequence-145756-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mammalian expression of HCMV protein UL136. Expresses 
            only the 26kda isoform of UL136. Also expresses EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG1_UL136_26kDa_Myc
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             626..649
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             662..685
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
       61 tagagctagc gaattcacca tgaccgcggt gtttcacgtt atctgtgccg ttttgcttac
      121 gcttgcgatt gcggccatcg gcgcgctcat cgcgtactta agatattacc accaggacag
      181 ttggcgagac gcgctccacg atctattttg cggctgtcat tatcctgaga agtgccgtcg
      241 gcaccacgag cggcagagaa gcagacggcg agccgcggat gtgcccgacc cggaactcgg
      301 cgacccggcc cgccggccgt tgaacggggc catgtactac ggcagcggct gtcgcttcga
      361 cacggtggaa atggtggacg agacgagacc cgcgccgccg gcgctgtcat cgcccgaaac
      421 cggcgacgat agcaacgacg acgcggttgc cggcggaggt gctggcgggg taacatcatc
      481 cgcgactcgt acgacgtcgt cgaacgcgct gctgccagaa tggatggatg cggtacatgt
      541 ggcggtccaa gccgccgttc aagcgaccgt gcaagtaagt ggcccgcggg agaacgccgt
      601 atctcccgct acgggcggtg gcggttggag ccacccgcag ttcgaaaaag gcggtggcgg
      661 ttggagccac ccgcagttcg aaaaataagg atccaattcc gc
//
