LOCUS       sequence-145764-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mammalian expression of HCMV protein UL136. Expresses 
            only the 33kda isoform of UL136. Also expresses EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG1_UL136_33kda_Myc
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             808..837
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagatt ctagagctag cgaattcatg tcagtcaagg gcgtggagat gccagaaatg
      121 acgtgggact tggacgttgg aaataaatgg cggcgtcgaa aggccctgag tcgcattcac
      181 cggttctggg aatgtcgact acgggtgtgg tggctgagtg acgccggcgt aagagaaacc
      241 gacccaccgc gtccccgacg ccgcccgact tgggcgaccg cggtgtttca cgttatctgt
      301 gccgttttgc ttacgcttgc gattgcggcc atcggcgcgc tcatcgcgta cttaagatat
      361 taccaccagg acagttggcg agacgcgctc cacgatctat tttgcggctg tcattatcct
      421 gagaagtgcc gtcggcacca cgagcggcag agaagcagac ggcgagccgc ggatgtgccc
      481 gacccggaac tcggcgaccc ggcccgccgg ccgttgaacg gggccatgta ctacggcagc
      541 ggctgtcgct tcgacacggt ggaaatggtg gacgagacga gacccgcgcc gccggcgctg
      601 tcatcgcccg aaaccggcga cgatagcaac gacgacgcgg ttgccggcgg aggtgctggc
      661 ggggtaacat catccgcgac tcgtacgacg tcgtcgaacg cgctgctgcc agaatggatg
      721 gatgcggtac atgtggcggt ccaagccgcc gttcaagcga ccgtgcaagt aagtggcccg
      781 cgggagaacg ccgtatctcc cgctacggaa caaaaactca tctcagaaga ggatctgtaa
      841 ggatccaatt ccg
//
