LOCUS       sequence-145770-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycyline regulated expression of Mesp2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Mesp2
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             651..659
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggcccagt cgcctcctcc tcagagcctc cagggtctcg accactgggt cttctcccag
       61 ggctggggct gggctcagca atcggactcc acgtctccgg cctcgtcctc agattcgtcc
      121 ggttcctgcc cttgctacgc cacccgtcgg ccctcgcagc ccgccggccc ggcccgtagc
      181 acgcgcacta cccaggcgac ggcgccccga cgaacgcgcc cagcgcccgc aggcggacag
      241 cggcagagcg ccagcgagcg cgagaagctg cgcatgcgca cactcgcccg cgcgctgcaa
      301 gaactgcgcc gcttcctgcc gccgtcggtg gcacctgcag gccagagcct gaccaagatc
      361 gagacgctgc gcctggccat ccgctacatc ggccacctgt cagccctgct gggcctcagc
      421 gaggacagtc tgcggcgcag gcgccgacgg agtgcggacg cggcgttctc tcaccgatgc
      481 cctcaatgcc ccgacggtgg cagcccctca caggctcaga tgcttggtcc tagcctggga
      541 tcagccatga gtagtggggt gtcctggggg tgcccgcctg cttgtcctgg acctctgatc
      601 tcacctgaaa accttgggaa caggatctcc aacgtggatc cctgggtgac acctccttat
      661 tgtccccaaa tacagtcacc cttacaccag tccctagaaa gagccgctga ctcctctccc
      721 tgggcaccac ctcaagcatg tcctggcatg cagatgtccc cagagcctag gaacaagact
      781 ggacactgga cacaatccac tgaacctgca gagctgacta aagtgtatca gagtctttct
      841 gtgtctccag aaccctgcct gtccctggga agcccacttc tcctgccccg cccatcatgc
      901 cagagactac agcctcagcc tcagcctcag cctcagtggg gctgctgggg ccacgatgca
      961 gaggtgctct ccacctctga ggatcagggt tccagccctg ccctccagct tcctgtggcc
     1021 agccccaccc ccagctcagg cctgcagctc agtggctgtc ctgaactttg gcaggaagac
     1081 ctggaaggac ccccactgaa tattttctac taa
//
