LOCUS       sequence-145791-        6221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bidirectional CAG promoter (compatible with the MXS Chaining Kit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors 
            in Embryonic Stem Cells.
  JOURNAL   PLoS One. 2016 May 6;11(5):e0155177. doi: 
            10.1371/journal.pone.0155177. eCollection 2016.
  PUBMED    27152616
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-05-6"; volume: "11(5):e0155177. doi"; pages: " 
            10.1371/journal.pone.0155177. eCollection 2016"
COMMENT     Sequence Label: MXS_BidirectionalCAG
FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          101..1117
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(1118..1394)
                     /label=chicken beta-actin promoter
     enhancer        1396..1775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        1783..2162
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2170..2549
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2557..2936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2946..3222
                     /label=chicken beta-actin promoter
     intron          3223..4239
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        4329..4433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4434..4502,4503..5294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4652..4671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5465..6053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5954..5973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgaagc ttacgcgtaa cctcgagcca tggaacgaat tctttgccaa
       61 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
      121 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg
      181 ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
      241 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
      301 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt
      361 tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata
      421 aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc
      481 gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct
      541 gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac
      601 ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga
      661 ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt
      721 tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca
      781 cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg
      841 cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc
      901 ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa
      961 caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc
     1021 tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg
     1081 agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt
     1141 tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct
     1201 gattggctgc cgccgcacct ctccgcctcg cccgccccgc ccctcgcccc gccccgcccc
     1261 gcctggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat
     1321 aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt
     1381 ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     1441 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     1501 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     1561 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     1621 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     1681 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     1741 tgattactat taataactag tcaataatca atgtcgagaa cccatggtaa tagcgatgac
     1801 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     1861 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     1921 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     1981 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2041 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     2101 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     2161 tcgagaaccc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     2221 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     2281 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     2341 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     2401 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     2461 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     2521 ctattaataa ctagtcaata atcaatgtcg agaacccatg gtaatagcga tgactaatac
     2581 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     2641 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     2701 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     2761 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     2821 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     2881 atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcgagc
     2941 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     3001 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     3061 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggc gaggcggaga
     3121 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     3181 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
     3241 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     3301 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
     3361 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
     3421 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     3481 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
     3541 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     3601 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     3661 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     3721 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     3781 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     3841 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     3901 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     3961 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     4021 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     4081 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     4141 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     4201 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     4261 attgtgctgt ctcatcattt tggcaaagaa ttcgtcgaca agcttctcga ccaattcggg
     4321 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4381 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4441 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4501 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4561 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4621 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4681 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4741 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4801 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4861 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4921 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4981 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5041 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5101 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5161 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5221 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5281 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5341 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5401 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5461 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5521 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5581 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5641 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5701 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5761 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5821 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5881 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5941 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6001 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6061 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag c
//
