LOCUS       sequence-145808-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mammalian expression of HCMV protein UL136. Expresses 
            only the 25kda isoform of UL136. Also expresses EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG1_UL136_25kDa_Myc
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             596..619
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             626..649
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             656..679
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gattctagag ctagcgaatt caccatgatt atggccatcg gcgcgctcat
      121 cgcgtactta agatattacc accaggacag ttggcgagac gcgctccacg atctattttg
      181 cggctgtcat tatcctgaga agtgccgtcg gcaccacgag cggcagagaa gcagacggcg
      241 agccgcggat gtgcccgacc cggaactcgg cgacccggcc cgccggccgt tgaacggggc
      301 catgtactac ggcagcggct gtcgcttcga cacggtggaa atggtggacg agacgagacc
      361 cgcgccgccg gcgctgtcat cgcccgaaac cggcgacgat agcaacgacg acgcggttgc
      421 cggcggaggt gctggcgggg taacatcatc cgcgactcgt acgacgtcgt cgaacgcgct
      481 gctgccagaa tggatggatg cggtacatgt ggcggtccaa gccgccgttc aagcgaccgt
      541 gcaagtaagt ggcccgcggg agaacgccgt atctcccgct acgggcggtg gcggtgatta
      601 taaagatgat gatgataaag gggcagacta caaggacgac gacgacaagg aattcgacta
      661 caaggacgac gacgacaagt aaggatccaa ttc
//
