LOCUS       sequence-145812-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RPU standard plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN1717
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(110..144)
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     primer_bind     complement(306..325)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(390..409)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ccatctagta tttcccctct ttctctagta ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagcttcagg ctagcataat
      121 acctaggact gagctagctg taaaagttag ggacttatca agcgggagac cagaaacaaa
      181 aaaaggcccc ccgttaggga ggccttcaat aattggttgt gtctcaaaat ctctgatgta
      241 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
      301 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcca ggccgcgatt
      361 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
      421 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
      481 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
      541 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
      601 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
      661 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
      721 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
      781 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
      841 tgaacaagtc tggaaagaa
//
