LOCUS       sequence-145814-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mammalian expression of HCMV protein UL136. Expresses 
            only the 26kda isoform of UL136. Also expresses EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F
  TITLE     Complex expression of the UL136 gene of human cytomegalovirus 
            results in multiple protein isoforms with unique roles in 
            replication.
  JOURNAL   J Virol. 2014 Dec;88(24):14412-25. doi: 10.1128/JVI.02711-14. Epub 
            2014 Oct 8.
  PUBMED    25297993
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.02711-14"; journalName: "J Virol"; date: "2014-12"; 
            volume: "88"; issue: "24"; pages: "14412-25"
COMMENT     Sequence Label: pCIG1_UL136_26kDa_Myc
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             630..653
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             666..689
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
       61 attctagagc tagcgaattc accatgaccg cggtgtttca cgttatctgt gccgttttgc
      121 ttacgcttgc gattgcggcc atcggcgcgc tcatcgcgta cttaagatat taccaccagg
      181 acagttggcg agacgcgctc cacgatctat tttgcggctg tcattatcct gagaagtgcc
      241 gtcggcacca cgagcggcag agaagcagac ggcgagccgc ggatgtgccc gacccggaac
      301 tcggcgaccc ggcccgccgg ccgttgaacg gggccatgta ctacggcagc ggctgtcgct
      361 tcgacacggt ggaaatggtg gacgagacga gacccgcgcc gccggcgctg tcatcgcccg
      421 aaaccggcga cgatagcaac gacgacgcgg ttgccggcgg aggtgctggc ggggtaacat
      481 catccgcgac tcgtacgacg tcgtcgaacg cgctgctgcc agaatggatg gatgcggtac
      541 atgtggcggt ccaagccgcc gttcaagcga ccgtgcaagt aagtggcccg cgggagaacg
      601 ccgtatctcc cgctacgggc ggtggcggtt ggagccaccc gcagttcgaa aaaggcggtg
      661 gcggttggag ccacccgcag ttcgaaaaat aag
//
