LOCUS       sequence-145818-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v4-kif18b-24xPP7
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             142..198
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             214..270
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             286..342
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             358..414
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             430..486
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             502..558
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             574..630
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             646..702
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             718..774
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             790..846
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             862..918
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             934..990
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc gtttaaactt
       61 aagcttgcca ccatgggcgc catcatacac gcggccgcaa tcatcggtcc gggtggatct
      121 ggaggtggag gttctggagg agaagaactt ttgagcaaga attatcatct tgagaacgaa
      181 gtggctcgtc ttaagaaagg ttctggcagt ggagaagaac tgctttcaaa gaattaccac
      241 ctggaaaatg aggtagctag actgaaaaag gggagcggaa gtggggagga gttgctgagc
      301 aaaaattatc atttggagaa cgaagtagca cgactaaaga aagggtccgg atcgggtgag
      361 gagttactct cgaaaaatta tcatctcgaa aacgaagtgg ctcggctaaa aaagggcagt
      421 ggttctggag aagagctatt atctaaaaac taccacctcg aaaatgaggt ggcacgctta
      481 aaaaagggaa gtggcagtgg tgaagagcta ctatccaaga attatcatct tgagaacgag
      541 gtagcgcgtt tgaagaaggg ttccggctca ggagaggaac tgctctcgaa gaactatcat
      601 cttgaaaatg aggtcgctcg attaaaaaag ggatcgggca gtggtgagga actactttca
      661 aagaattacc acctcgaaaa cgaagtagct cgattaaaga aaggttcagg gtcgggtgaa
      721 gaattactga gtaaaaatta tcatctggaa aatgaggtag cgagactaaa aaaggggagt
      781 ggttctggcg aagagttgct atcgaaaaat tatcatcttg agaacgaagt tgctaggctc
      841 aaaaagggct caggctcagg cgaggagttg ctctcgaaaa actaccactt ggaaaatgag
      901 gtcgcgaggt tgaaaaaggg gagcgggtcg ggcgaggagt tattgagcaa aaactatcat
      961 ttagagaacg aagtcgcgcg cttaaagaaa ggctcgggct cgggcga
//
