LOCUS       sequence-145827-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) 
            via a T2A motif. For cell transfection or use in lentiviral 
            packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto 
            CP, Geller JS, Li M, Luikart BW
  TITLE     A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated 
            Phenotype Discovery in Developing Neurons.
  JOURNAL   Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
  PUBMED    27161796
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-10"; volume: "6:25611. doi"; pages: " 
            10.1038/srep25611"
COMMENT     Sequence Label: FUG-T2A-Cas9
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..98)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattctta ctttttcttt
       61 tttgcctggc cggccttttt cgtggccgcc ggccttttgt cgcctcccag ctgagacagg
      121 tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc gtccagcacc
      181 tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt gaaggcggca
      241 ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg ctctctgatg
      301 ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc gtcggccagg
      361 atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta gtgcttgtgc
      421 tgttccacaa acagctgttt ctgctcatta tcctcggggg agcccttcag cttctcatag
      481 tggctggcca ggtacaggaa gttcacatat ttggagggca gggccagttc gtttcccttc
      541 tgcagttcgc cggcagaggc cagcattctc ttccggccgt tttccagctc gaacagggag
      601 tacttaggca gcttgatgat caggtccttt ttcacttctt tgtagccctt ggcttccaga
      661 aagtcgatgg gattcttctc gaagctgctt ctttccatga tggtgatccc cagcagctct
      721 ttcacactct tcagtttctt ggacttgccc ttttccactt tggccaccac cagcacagaa
      781 taggcc
//
