LOCUS       sequence-145830-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      475..484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             523..579
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             595..651
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             667..723
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             739..795
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             811..867
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag cgtttaaact
       61 taagcttgtt aggagggaga gcagagcgcg cgcgtaaatc ctagagaggc gggctaagct
      121 ggactggggg gagggtccgt cttccggaaa gtctggattc ccggacgagc cgagttgctg
      181 ctcaccgaac tcccgttcga gagatgatcg aagaaagtcg gctaccattt gtacccatca
      241 aagatctcca gatggaagcc agcgctgaat ttgggctgag attaggactt gcaggaggcc
      301 ggtccagaag acggcggaag gaatcttggc gggcgcacgc atgcgtgata gaccctccac
      361 acgtgtggcc gggccgcggc ctccccgtgc tcggaggtcc cgcccccggc cgtagcatct
      421 ttccggacgt ggggagccgg ttgtaaagaa gaaagttcta ccctttctgt caaagccacc
      481 atggtcggtc cgggtggatc tggaggtgga ggttctggag gagaagaact tttgagcaag
      541 aattatcatc ttgagaacga agtggctcgt cttaagaaag gttctggcag tggagaagaa
      601 ctgctttcaa agaattacca cctggaaaat gaggtagcta gactgaaaaa ggggagcgga
      661 agtggggagg agttgctgag caaaaattat catttggaga acgaagtagc acgactaaag
      721 aaagggtccg gatcgggtga ggagttactc tcgaaaaatt atcatctcga aaacgaagtg
      781 gctcggctaa aaaagggcag tggttctgga gaagagctat tatctaaaaa ctaccacctc
      841 gaaaatgagg tggcacgctt aaaaaaggga agtggcagtg gtgaagagct actatccaag
      901 aattatcatc ttgagaacga ggta
//
