LOCUS       sequence-145834-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) 
            via a T2A motif. For cell transfection or use in retroviral (MMuLV) 
            packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto 
            CP, Geller JS, Li M, Luikart BW
  TITLE     A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated 
            Phenotype Discovery in Developing Neurons.
  JOURNAL   Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
  PUBMED    27161796
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-10"; volume: "6:25611. doi"; pages: " 
            10.1038/srep25611"
COMMENT     Sequence Label: pRubiC-T2A-Cas9
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(290..308)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     558..577
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
       61 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt gggctgcagg
      121 tcgactctag aggatcccgc caccatggtg agcaagggcg aggaggataa catggccatc
      181 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      241 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      301 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      361 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      421 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      481 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      541 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
      601 gagcggatgt accccgatga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
      661 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      721 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
//
