LOCUS       sequence-145835-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) 
            via a T2A motif. For cell transfection or use in retroviral (MMuLV) 
            packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto 
            CP, Geller JS, Li M, Luikart BW
  TITLE     A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated 
            Phenotype Discovery in Developing Neurons.
  JOURNAL   Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
  PUBMED    27161796
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-10"; volume: "6:25611. doi"; pages: " 
            10.1038/srep25611"
COMMENT     Sequence Label: pRubiC-T2A-Cas9
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..99)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt actttttctt
       61 ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tcgcctccca gctgagacag
      121 gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac
      181 ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc
      241 aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat
      301 gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag
      361 gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg
      421 ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata
      481 gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt
      541 ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga
      601 gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct tggcttccag
      661 aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc
      721 tttcacactc ttcagtttct tggacttgcc ctt
//
