LOCUS       sequence-145836-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ZNF219 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Mendenhall CRISPR tagging plasmids
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFETCh_ZNF219
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..109)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(125..148)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(152..175)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(179..202)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccaggtccag
       61 ggttctcctc cacgtctcca gcctgcttca gcaggctgaa gttagtagct ccgcttcctg
      121 aaaccttatc gtcgtcatcc ttgtaatctc ccttatcgtc gtcatccttg taatcgccct
      181 tatcgtcgtc atccttgtaa tctccagaac cacctccacc ggaacctcct ccgctctccc
      241 gttcttgccc ccccagccct gcctctccgg gtctggacag cccggagccc tcctcccctt
      301 cctgcgaagg actgggaggg gtctctcctg atggtactcg ggcatagggc ggggacgcgt
      361 cagcctgggg tggccggcgg cccctagccc ggcggctgtg gtgcacttga aggtgcaagg
      421 ccatgagctc tggggctcca gtggcgaacg ggcagaagag gcagcggtgg agggcacccc
      481 caggcccggc ctcgcctccc ggccctgccc gcaaggacag gtccaggggt tcggcctcac
      541 cgcctcgccc gttgcgcagg gtcctcccag ggctggcggg cttccgacgg gaccccggcc
      601 cagcaccgct agaaggaggc cggggacttg aggcgccctc cacccaggtc gcaggctgcg
      661 gagacggctt ggctccagat tgcggggccg aaccccgctg ggaaggaggc ggtggctccg
      721 ggggtggccc ggggccggcc ccgctcctct gctcccggtg gtggcgctgt aggtgatact
      781 tgagcgagcc ggactgggtg cccgcgtagt cgcagtgcgg acacttgtag ggccgctcgc
      841 ctggggagag tgggtgcgat agggtcacgg ggtcacgatg actgcacgca cccacccgaa
      901 tttcgccc
//
