LOCUS       sequence-145839-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a 
            T2A motif. For cell transfection or use in retroviral (MMuLV) 
            packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto 
            CP, Geller JS, Li M, Luikart BW
  TITLE     A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated 
            Phenotype Discovery in Developing Neurons.
  JOURNAL   Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
  PUBMED    27161796
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-10"; volume: "6:25611. doi"; pages: " 
            10.1038/srep25611"
COMMENT     Sequence Label: pRubiG-T2A-Cas9
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..89)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tccagaggtt gattatcgat aagcttgata tcgaattctt actttttctt ttttgcctgg
       61 ccggcctttt tcgtggccgc cggccttttg tcgcctccca gctgagacag gtcgatccgt
      121 gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg
      181 ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc
      241 agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat gggcttatcc
      301 cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag gatcactctc
      361 ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg ctgttccaca
      421 aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata gtggctggcc
      481 aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt ctgcagttcg
      541 ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga gtacttaggc
      601 agcttgatga tcaggtcctt tttcacttct ttgtagccct tggcttccag aaagtcgatg
      661 ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc tttcacactc
      721 ttcagtttct tggacttgcc ctt
//
