LOCUS       sequence-145844-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-5xGCN4_v4-kif18b-24xPP7
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             120..176
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             192..248
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             264..320
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             336..392
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             408..464
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
       61 agcttgccac catgggcgcc atcatacacg cggccgcaat catcggtccg ggttctggag
      121 aagagctact atccaagaat tatcatcttg agaacgaggt agcgcgtttg aagaagggtt
      181 ccggctcagg agaggaactg ctctcgaaga actatcatct tgaaaatgag gtcgctcgat
      241 taaaaaaggg atcgggcagt ggtgaggaac tactttcaaa gaattaccac ctcgaaaacg
      301 aagtagctcg attaaagaaa ggttcagggt cgggtgaaga attactgagt aaaaattatc
      361 atctggaaaa tgaggtagcg agactaaaaa aggggagtgg ttctggcgag gaattgctat
      421 cgaaaaatta tcatcttgag aacgaagttg ctaggctcaa aaagggctca ggctcaggca
      481 ccgcggtaaa catatggatc gatcagcggc cgcaccggtc agcagtggag gacagcacgc
      541 tgcaagtagt ggtacgggtg cggcccccca cccctcggga gctggacagt cagcggcggc
      601 cagtggttca ggtggtggac gagcgggtgc tggtgtttaa ccctgaggag cccgatggag
      661 ggttccctgg cctgaaatgg ggtggcaccc atgatggccc caagaagaag ggcaaagacc
      721 tgacgtttgt ctttgaccgg gtctttggcg aggcggccac ccaacaggac gtgttccagc
      781 acaccacgca cagcgtcctg gacagcttcc tccagggcta caactgctca gtgtttgcct
      841 acggggccac cggggctggg aagacacaca ccatgctggg aagggagggg gaccccggca
      901 tcatgtacct gaccaccgtg gaactgtaca ggcgcctgga ggcccgccag caggagaagc
      961 acttcgaggt gctcatcagc taccaggagg tgtataatga acagatccat gacctcctgg
     1021 ag
//
