LOCUS       sequence-145847-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PX330 derived. Bbs1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto 
            CP, Geller JS, Li M, Luikart BW
  TITLE     A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated 
            Phenotype Discovery in Developing Neurons.
  JOURNAL   Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
  PUBMED    27161796
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-10"; volume: "6:25611. doi"; pages: " 
            10.1038/srep25611"
COMMENT     Sequence Label: PXL
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..96
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             103..123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttttttcag gttggaccgg tgccaccatg gactataagg accacgacgg agactacaag
       61 gatcatgata ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag
      121 gtcggtatcc acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc
      181 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
      241 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
      301 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
      361 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
      421 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
      481 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
      541 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
      601 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
      661 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
      721 aaccagctg
//
