LOCUS       sequence-145848-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PX330 derived. Bbs1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Williams MR, Fricano-Kugler CJ, Getz SA, Skelton PD, Lee J, Rizzuto 
            CP, Geller JS, Li M, Luikart BW
  TITLE     A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated 
            Phenotype Discovery in Developing Neurons.
  JOURNAL   Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
  PUBMED    27161796
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-05-10"; volume: "6:25611. doi"; pages: " 
            10.1038/srep25611"
COMMENT     Sequence Label: PXL
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..136
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        213..288
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
       61 tagcaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      121 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      181 aaggacgaaa caccgggtct tcgagaagac ctgttttaga gctagaaata gcaagttaaa
      241 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      301 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
      361 cagacaaatg gctctagggt taattaaggt ctagaggtac ccgttacata acttacggta
      421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat agtaacgcca
      481 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
//
