LOCUS       sequence-145851-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expressing YFP in mammalian cells upon Cre co-expression. Can be
            made into Lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU - dio YFP - W
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(64..97)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(148..181)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(194..196,197..199,200..913)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(239..260)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(500..519)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     847..868
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    922..955
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa
       61 ttcataactt cgtatagcat acattatacg aagttattct ttgcaccatt ctaaagaata
      121 acagtgataa tttctgggtt aaggcaaata acttcgtata ggatacttta tacgaagtta
      181 tgctagcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      361 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
      421 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      601 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      661 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      781 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
      841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      901 gctgtacaag taaggcgcgc cataacttcg tataatgtat gctatacgaa gttattaaga
      961 ggtttcatat tgctaatagc agctacaatc cagctaccat tctgcataac ttcgtataaa
     1021 gtatcctata c
//
