LOCUS       sequence-145860-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the first coiled-coil domain of mouse ninein with an EGFP 
            tag at its N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Delgehyr N, Sillibourne J, Bornens M
  TITLE     Microtubule nucleation and anchoring at the centrosome are 
            independent processes linked by ninein function.
  JOURNAL   J Cell Sci. 2005 Apr 15;118(Pt 8):1565-75. Epub 2005 Mar 22.
  PUBMED    15784680
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2005-04-15"; volume: "118(Pt 8)"; pages: "1565-75. 
            Epub 2005 Mar 22"
COMMENT     Sequence Label: pEGFP-ninein coiled-coil 1
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(56..259)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(85..105)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        260..563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ttgctcacca tggtggcgac cggtagcgct agcggatctg acggttcact aaaccagctc
       61 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
      121 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
      181 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
      241 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
      301 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
      361 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
      421 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
      481 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
      541 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
      601 taattgatta ctattaataa ctaatgcatg gcggtaatac ggttatccac agaatcaggg
      661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      721 gccgcgttgc tggcgttttt ccataggctc cgcc
//
