LOCUS       sequence-145886-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APEX2 in mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: mito-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(8..436)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(118..139)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     330..349
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    482..706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     695..712
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        732..750
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     732..749
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
       61 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
      121 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
      181 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
      241 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
      301 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
      361 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
      421 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
      481 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
      541 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
      601 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
      661 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
      721 cgagctctag catttaggtg acactataga atagggccct ctagatgcat gctcgagtca
      781 ttaggcatca gcaaacccaa gctcggaaag cttttggtga gcctcagcgt aatcagcaaa
      841 gaaggcatct tcgtccgctg catatttatc aacgagaggg cggaatacag ggtcagacaa
      901 aagagccttg tcagaaggta gctgaaggag accttccttc tcaccactca acaactccgt
      961 gaagtatgag ttgtcgaaaa taagaggatt agaggtccag g
//
