LOCUS       sequence-145894-       11280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector encoding Mars1Cy-eGFP fusion for 
            intracellular expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Zhang M, Chakraborty SK, Sampath P, Rojas JJ, Hou W, Saurabh S, 
            Thorne SH, Bruchez MP, Waggoner AS
  TITLE     Fluoromodule-based reporter/probes designed for in vivo fluorescence
            imaging.
  JOURNAL   J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. 
            Epub 2015 Sep 8.
  PUBMED    26348895
REFERENCE   2  (bases 1 to 11280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI81086"; 
            journalName: "J Clin Invest"; date: "2015-10-1- 1"; volume: "125"; 
            issue: "10"; pages: "3915-27"
COMMENT     Sequence Label: pLenCMV-Mars1CyeGFP
FEATURES             Location/Qualifiers
     source          1..11280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2662..2965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2966..3169
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3208..3217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3988..3990,3991..3993,3994..4707)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3988..3990,3991..3993,3994..4704)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4033..4054)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4294..4313)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4641..4662
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4740..5313,5314..5316,5317..5325)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4907..4924)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5134..5153
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5345..5944
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5345..5364)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5841..5861
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5972..6560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6025..6045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6443..6454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6563..6579)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6564..6580)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7085..7265
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7291..7308)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7297..7521
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7567..7995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7654..7673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7864..7885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8004..8024)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8009..8338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8189..8324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8251..8270
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8386..8433
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8452..8826
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8956..9077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8993..9012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9047..9066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9126..9142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9126..9142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9139..9161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9150..9166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9174..9180,9181..9198,9199..9204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9219..9240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9357..9374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9528..10116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9608..9627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10287..11078,11079..11147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10910..10929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11148..11252)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atagttatta atagtaatca attacggggt cattagttca
     2641 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2701 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2761 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2821 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2881 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2941 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3001 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3061 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     3121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     3181 ccgtcagatc cgctagcgct accggtcgcc accatggcct cttacgaatt aacacaacct
     3241 ccatctgttt ctgtttctcc aggtcaaact gctagaatta cttgttctgg tgatgctttg
     3301 ccaaaacaat acacttactg gtatcaacaa aaagctggtc aagctccagt tttggttatt
     3361 tataaagata ctgaaagacc atctggtatt ccagaaagat tctcaggtac ttcttctggt
     3421 actactgcta ctttgactat ttctggtgtt caagctgaag atgaagctga ttattattgt
     3481 caatctgctg attcttctgg ttctcatgta tttttcggtg gtggtactaa agttactgtt
     3541 ttgtctggtg gaggaggccc aggtggcggt tcaggtggag gttctggcgg aggttctggt
     3601 ggaggttcct atgagttgac tcagccaccc tcggtgtcag tgtccccagg acagacggcc
     3661 aggatcacct gctctggaga tgcattgcca aagcaatata cttattggta ccagcagaag
     3721 gcaggccagg cccctgtctt ggtgatatat aaagacactg agaggccctc agggatccct
     3781 gagcgattct ctggtaccag ttcagggaca acagccacat tgaccatcag tggagtccag
     3841 gcagaagacg aggctgacta ttactgtcaa tcagcagaca gcagtggttc ccacgttttc
     3901 ttcggcggag ggaccaaggt gaccgtacta tccggagggc gcgcctctgg tggcggagct
     3961 tctggaggtg gttctggcgg ctctggtatg gtgagcaagg gcgaggagct gttcaccggg
     4021 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4081 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4141 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4201 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4261 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4321 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4381 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4441 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4501 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4561 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4621 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4681 ctcggcatgg acgagctgta taagtaatgt acatagtaat taacgcatcc ggatttccgc
     4741 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4801 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4861 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4921 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4981 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5041 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5101 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5161 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5221 acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg
     5281 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     5341 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     5401 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     5461 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5521 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5581 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5641 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5701 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5761 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5821 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5881 cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca agcccggtgc
     5941 ctgataggaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     6001 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6061 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6121 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6181 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6241 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6301 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6361 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6421 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6481 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6541 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     6601 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     6661 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     6721 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6781 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     6841 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     6901 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     6961 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     7021 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     7081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7261 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     7321 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7381 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7441 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7501 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     7561 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7621 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7681 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7741 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7801 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7861 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7921 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7981 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8041 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8101 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8161 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8221 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     8281 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8341 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     8401 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     8461 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     8521 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     8581 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     8641 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     8701 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     8761 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     8821 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8881 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8941 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9061 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     9121 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9181 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9241 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9301 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9361 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9421 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
