LOCUS       sequence-145912-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Over-expression of human KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-PGK-hKLF4
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..44
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(59..64,65..82,83..89)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    97..113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     102..124
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     121..137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     121..137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    158..182
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
       61 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      121 caggaaacag ctatgaccat gattacgcca agctatcacc actttgtaca agaaagctgg
      181 gtcggcgcgc ccaccctttt aaaaatgtct cttcatgtgt aaggcgaggt ggtccgacct
      241 ggaaaatgct cggtcgcatt tttggcactg gaacgggcgg tgccccgtgt gtttacggta
      301 gtgcctggtc agttcatctg agcgggcgaa tttccatcca cagccgtccc agtcacagtg
      361 gtaaggtttc tcacctgtgt gggttcgcag gtgtgccttg agatgggaac tctttgtgta
      421 ggttttgccg cagcccgcgt aatcacaagt gtgggtggcg gtccttttcc ggggccacga
      481 tcgtcttccc ctctttggct tgggctcctc tggcatgcag gaaccgggtg gcatgagctc
      541 ttggtaatgg agcggcggga cttgcggctg catctgatcg ggcaggaagg atgggtaatt
      601 gggccccggg tggggatgga agccgggagg aagcggcagg gcagggtgac agtccctgct
      661 gctcagcact tcctcaagac ccagggtcgg ggtagtcctg ctggggagct gccgccccag
      721 ggggaagtcg tgtgcagccg gccggtggcc attgctgaga gggggtccag cgcccaagtg
      781 ggtgcacgaa gagaccgcct cctgcttgat cttggggcac gtgcgcggcg gcccgccgtt
      841 gtagggcgcc accac
//
