LOCUS       sequence-145916-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone for tet-inducible genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-CAG-rtTA-IN
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(97..714,715..720,721..756,757..759,760..795,796..798,
                     799..834,835..843)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
       61 tctcatcatt ttggcaaaga attcctcgag gccaccatgt ctagactgga caagagcaaa
      121 gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct gacgacaagg
      181 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
      241 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
      301 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc attccgctgt
      361 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
      421 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
      481 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
      541 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
      601 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
      661 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
      721 gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
      781 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
      841 taagcggccg ctcgataa
//
