LOCUS       sequence-145917-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CRY2(1-498)-mCherry fusion for use in light-inducible 
            protein interaction modules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pCRY2PHR-mCherryN1
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
       61 tggccatgtt atcctcctcg cccttgctca ccatggtggc gaccggtgga tcccgggctg
      121 ctgctccgat catgatctgt gcttcacggg ttcttgaaat agctttagct agtagctcac
      181 gagctgtgtc gatgtctaca atgggtttcg catagtttgt tccgagttcc acaccagaag
      241 ctttgagtac ggttaaagga gcgtcccatg gatgatggat ccattcagtt ggcaatctcg
      301 caagctcggg aagccattgc cttatgtact caccttctgg gtcatatttg gcgccttgta
      361 acgcgggatt gtccaagcga tcaagctcgt ggccatcggg gatactccca gagatatact
      421 gccagccaag gatgtcacat tccaaatcag catccaaaag tgtatcccag aaatacttca
      481 ttccccattt ccatggaagg agaagaaact tcacagcaaa gcttgaaaca atcactctta
      541 ttctgttatg catccatccg gtagcccaaa gctctctcat tccggcatcc accaacggat
      601 aaccggtcct gccttgtctc caggccttga acttatcaac atcagcatcc caagggaaaa
      661 accgaagatg actcaacaac gattgctcgt gagtaaacgg gaagttgaaa catatatacc
      721 gagaatactc tcttaaaccg attcc
//
