LOCUS       sequence-145927-        6621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HIV reverse transcriptase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Elbaz J, Yin P, Voigt CA
  TITLE     Genetic encoding of DNA nanostructures and their self-assembly in 
            living bacteria.
  JOURNAL   Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179.
  PUBMED    27091073
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-19"; volume: "7:11179. doi"; pages: " 
            10.1038/ncomms11179"
COMMENT     Sequence Label: pHIV_pTp66p51
FEATURES             Location/Qualifiers
     source          1..6621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(91..120)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(183..1265)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1227..1246
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1400..1405,1406..1421,1422..1428)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1434..1453
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_RNA        1469..1518
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             1527..1538
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             3239..3250
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4605..4676
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4692..4719
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(4752..4769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4923..5511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5003..5022)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5682..6473,6474..6542))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(6543..6621,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc
      121 agtgagttga ttgctacgta atgtcggcca attcgcgcta acttacatta attgcgttgc
      181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      241 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
      301 gagactggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
      361 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
      421 taacatgagc tatcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
      481 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
      541 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
      601 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
      661 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
      721 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg
      781 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
      841 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
      901 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
      961 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     1021 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     1081 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     1141 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     1201 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     1261 ttcatattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     1321 gttttgcgcc attcgatggc gcgccgcttc gtcaggccac atagctttct tgttctgatc
     1381 ggaacgatcg ttggctgtgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
     1441 agcgctcaca attctatgga ctatgtttct gtcaccggat gtgctttccg gtctgatgag
     1501 tccgtgagga cgaaacagta ctagtgaaag aggagaaata ctagatgccg attagcccga
     1561 ttgaaaccgt tccggttaaa ctgaaaccgg gtatggatgg tccgaaagtt aaacagtggc
     1621 ctctgaccga agaaaaaatc aaagcactgg ttgaaatctg caccgagatg gaaaaagaag
     1681 gcaaaattag caaaatcggt ccggaaaatc cgtataatac accggttttt gccattaaga
     1741 aaaaagatag caccaaatgg cgcaaactgg tggattttcg tgaactgaat aaacgcaccc
     1801 aggatttttg ggaagttcag ctgggtattc cgcatccggc aggtctgaaa cagaaaaaaa
     1861 gcgttaccgt tctggatgtt ggtgatgcat attttagcgt tccgctggat aaagatttcc
     1921 gtaaatatac cgcatttacc atcccgagca ttaataacga aacaccgggt attcgctatc
     1981 agtataatgt tctgccgcag ggttggaaag gtagtccggc aatttttcag tgtagcatga
     2041 ccaaaattct ggaaccgttt cgtaaacaga atccggatat tgtgatctac cagtatatgg
     2101 atgatctgta tgttggtagc gatctggaaa ttggtcagca tcgtaccaaa attgaagaac
     2161 tgcgtcagca tctgctgcgt tggggtttta ccacaccgga taaaaaacat cagaaagaac
     2221 cgccttttct gtggatgggt tatgaactgc atccggataa atggaccgtt cagccgattg
     2281 ttctgccgga aaaagatagc tggaccgtta atgatattca gaaactggtg ggtaaactga
     2341 attgggcaag ccagatttat gccggtatta aagttcgtca gctgtgtaaa ctgctgcgtg
     2401 gcaccaaagc actgaccgaa gttgttccgc tgacagaaga agcagaactg gaactggcag
     2461 aaaatcgtga aattctgaaa gaaccggttc acggcgttta ttatgatccg agcaaagatc
     2521 tgattgccga aattcagaaa cagggtcagg gtcagtggac ctatcagatt tatcaagaac
     2581 cgtttaaaaa cctgaaaacc ggcaaatatg cacgtatgaa aggtgcacat accaacgatg
     2641 ttaaacagct gaccgaagca gttcagaaaa ttgcaaccga aagcattgtg atttggggta
     2701 aaaccccgaa attcaaactg ccgattcaga aagaaacctg ggaagcatgg tggaccgaat
     2761 attggcaggc aacctggatt ccggaatggg aatttgttaa tacccctccg ctggttaaac
     2821 tgtggtatca gctggaaaaa gaaccgatta ttggtgccga aaccttttat gttgatggtg
     2881 cagccaatcg tgaaaccaaa ctgggtaaag caggttatgt taccgatcgt ggtcgtcaga
     2941 aagtggtgcc gctgaccgat accaccaatc agaaaaccga actgcaggca attcatctgg
     3001 cactgcagga tagcggtctg gaagttaata ttgttaccga tagccagtat gccctgggta
     3061 ttattcaggc acagccggat aaaagcgaaa gcgaactggt tagccagatt attgaacagc
     3121 tgatcaaaaa agaaaaagtg tacctggcat gggttccggc acataaaggt attggtggta
     3181 atgaacaggt tgatggtctg gttagcgcag gtattcgtaa agttctgtaa tactagtgaa
     3241 agaggagaaa tactagatgc cgattagccc gattgaaacc gttccggtta aactgaaacc
     3301 gggtatggat ggtccgaaag ttaaacagtg gcctctgacc gaagaaaaaa tcaaagcact
     3361 ggttgaaatc tgcaccgaga tggaaaaaga aggcaaaatt agcaaaatcg gtccggaaaa
     3421 tccgtataat acaccggttt ttgccattaa gaaaaaagat agcaccaaat ggcgcaaact
     3481 ggtggatttt cgtgaactga ataaacgcac ccaggatttt tgggaagttc agctgggtat
     3541 tccgcatccg gcaggtctga aacagaaaaa aagcgttacc gttctggatg ttggtgatgc
     3601 atattttagc gttccgctgg ataaagattt ccgtaaatat accgcattta ccatcccgag
     3661 cattaataac gaaacaccgg gtattcgcta tcagtataat gttctgccgc agggttggaa
     3721 aggtagtccg gcaatttttc agtgtagcat gaccaaaatt ctggaaccgt ttcgtaaaca
     3781 gaatccggat attgtgatct accagtatat ggatgatctg tatgttggta gcgatctgga
     3841 aattggtcag catcgtacca aaattgaaga actgcgtcag catctgctgc gttggggttt
     3901 taccacaccg gataaaaaac atcagaaaga accgcctttt ctgtggatgg gttatgaact
     3961 gcatccggat aaatggaccg ttcagccgat tgttctgccg gaaaaagata gctggaccgt
     4021 taatgatatt cagaaactgg tgggtaaact gaattgggca agccagattt atgccggtat
     4081 taaagttcgt cagctgtgta aactgctgcg tggcaccaaa gcactgaccg aagttgttcc
     4141 gctgacagaa gaagcagaac tggaactggc agaaaatcgt gaaattctga aagaaccggt
     4201 tcacggcgtt tattatgatc cgagcaaaga tctgattgcc gaaattcaga aacagggtca
     4261 gggtcagtgg acctatcaga tttatcaaga accgtttaaa aacctgaaaa ccggcaaata
     4321 tgcacgtatg aaaggtgcac ataccaacga tgttaaacag ctgaccgaag cagttcagaa
     4381 aattgcaacc gaaagcattg tgatttgggg taaaaccccg aaattcaaac tgccgattca
     4441 gaaagaaacc tgggaagcat ggtggaccga atattggcag gcaacctgga ttccggaatg
     4501 ggaatttgtt aatacccctc cgctggttaa actgtggtat cagctggaaa aagaaccgat
     4561 tattggtgcc gaaacctttt aagatcgcta ctagagccag gcatcaaata aaacgaaagg
     4621 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     4681 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaagcggccg
     4741 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     5401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6601 tacatatttg aatgtattta g
//
