LOCUS       sequence-145935-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses LIC1 in mammalian cells; aa 389-523, untagged but cells 
            indicate expression with diffuse mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pHR-mCherry-p2A-LIC1 C-terminal half
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             277..333
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             751..777
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(899..919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
       61 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      121 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      181 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      241 ggcatggacg agctgtacaa gggatccgga agcggagcta ctaacttcag cctgctgaag
      301 caggctggag acgtggagga gaaccctgga cctcggtccg aaatgactgc agctggaagg
      361 cctgtggatg cctcaccaag agtcccagga ggctccccac gaacaccaaa tagatctgta
      421 tcatctaatg ttgccagcgt gtcacccatt cctgctgggt caaaaaaaat tgatccaaac
      481 atgaaagctg gagctacaag tgaaggcgtt ctggcaaatt tcttcaacag tttgttgagt
      541 aaaaagactg gctctccagg aggccctggt gtgagtggtg gtagccctgc aggtggggct
      601 ggaggtggaa gcagtggttt accaccatcc accaaaaagt caggccagaa gcctgtctta
      661 gatgttcatg cagaactaga cagaattaca cgaaaaccag ttacagtttc tcccacaaca
      721 cctacatctc ctacggaagg agaagcttct tacccatacg acgtcccaga ctacgcttga
      781 catatggcgg ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta
      841 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      901 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      961 cccgtatggc tttcattttc tcctccttg
//
