LOCUS       sequence-145943-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses LIC1 in mammalian cells; HA-tagged; point mutants that 
            abrogate binding to dynein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             277..333
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
       61 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      121 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      181 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      241 ggcatggacg agctgtacaa gggatccgga agcggagcta ctaacttcag cctgctgaag
      301 caggctggag acgtggagga gaaccctgga cctcggtccg aaatggcggc cgtggggcga
      361 gtcggctcct tcggttcttc tccgccggga ttatcctcga cttacactgg cggccccttg
      421 ggcaacgaga tagcgtcggg caacggtggc gccgcggcag gcgacgacga ggacgggcag
      481 aacctttggt cctgcatcct cagcgaggtc tccacccgct cgcgctccaa gctccctgcg
      541 gggaagaacg tgctactgct gggtgaagat ggagctggaa aaacaagctt aataagaaaa
      601 attcagggaa tagaggagta taagaaagga agaggattgg aagctttggc cttaaatgtg
      661 catgatgaag acagggatga tcaaacaaga tgtaatgttg cgatcttaga tggagaccta
      721 tatcacaaag gcctccttaa attttcactg gatgccgtat ctctgaagga tactctagtt
      781 atgctggttg ttgacatgtc aaagccttgg actgctttgg attctttaca gaaatgggca
      841 agtgttgtta gagaacatgt tgacaaactg aaaatccctc ctgaagaaat gaaacaaatg
      901 gaacaaaagt tgattagaga cttccaagaa tatgtagagc caggagaaga cttcccggct
      961 tctccccaga gaagaaatac tgcgtcacaa gaagacaaag atgacagtgt agttttacct
     1021 ctg
//
