LOCUS       sequence-145952-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used for expression in E. coli and purification. N-terminal GST tag 
            and C-terminal Strep tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pGEX6P1-human full-length LIC1
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..35
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caaaatcgga tctggaagtt ctgttccagg ggcccctggg atccatggcg gccgtggggc
       61 gagtcggctc cttcggttct tctccgccgg gattatcctc gacttacact ggcggcccct
      121 tgggcaacga gatagcgtcg ggcaacggtg gcgccgcggc aggcgacgac gaggacgggc
      181 agaacctttg gtcctgcatc ctcagcgagg tctccacccg ctcgcgctcc aagctccctg
      241 cggggaagaa cgtgctactg ctgggtgaag atggagctgg aaaaacaagc ttaataagaa
      301 aaattcaggg aatagaggag tataagaaag gaagaggatt ggaatatttg tacttaaatg
      361 tgcatgatga agacagggat gatcaaacaa gatgtaatgt ttggatctta gatggagacc
      421 tatatcacaa aggcctcctt aaattttcac tggatgccgt atctctgaag gatactctag
      481 ttatgctggt tgttgacatg tcaaagcctt ggactgcttt ggattcttta cagaaatggg
      541 caagtgttgt tagagaacat gttgacaaac tgaaaatccc tcctgaagaa atgaaacaaa
      601 tggaacaaaa gttgattaga gacttccaag aatatgtaga gccaggagaa gacttcccgg
      661 cttctcccca gagaagaaat actgcgtcac aagaagacaa agatgacagt gtagttttac
      721 ctctgggtgc ggatacactt acacataact tgggcattcc agtactagta gtttgcacaa
      781 agtgtgatgc cattagtgta ttggagaaag aacatgacta cagagatgaa cattttgatt
      841 ttattcagtc acatatccgg aagttttgtt tacagtatgg tgcagcactt atttacactt
      901 cagtaaaaga aaacaaaaat atagacttag tatataaata catcgttcag aaactatatg
      961 gatttcccta taagattcct gctgttgttg tggaaaagga tgca
//
