LOCUS       sequence-145953-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used for expression in E. coli and purification. N-terminal GST tag 
            and C-terminal Strep tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pGEX6P1-human full-length LIC1
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..91)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 cgaaacgcgc gaggcagatc gtcagtcagt cacgatgcgg ccgctcgagt cgacccggga
       61 attcctattt ttcgaactgc gggtggctcc agccaccaga agcttctcct tccgtaggag
      121 atgtaggtgt tgtgggagaa actgtaactg gttttcgtgt aattctgtct agttctgcat
      181 gaacatctaa gacaggcttc tggcctgact ttttggtgga tggtggtaaa ccactgcttc
      241 cacctccagc cccacctgca gggctaccac cactcacacc agggcctcct ggagagccag
      301 tctttttact caacaaactg ttgaagaaat ttgccagaac gccttcactt gtagctccag
      361 ctttcatgtt tggatcaatt ttttttgacc cagcaggaat gggtgacacg ctggcaacat
      421 tagatgatac agatctattt ggtgttcgtg gggagcctcc tgggactctt ggtgaggcat
      481 ccacaggcct tccagctgca gttggtggtt gctttgctaa aagggactgt agcttcataa
      541 gaaacacctg atcatcttct gccataattt ccttctcatg tacaaacttt cgaacaggtg
      601 gtttagttat gatgtcttca aaattatctt ctgcttttaa tgtttgaaaa ttttcatgta
      661 atattcctat tttcttatca ttatcccacc ctgctggaat aaatactgca tccttttcca
      721 caacaacagc aggaatctta tagggaaatc catatagttt ctgaacgatg tatttatata
      781 ctaagtctat atttttgttt tcttttactg aagtgtaaat aagtgctgca ccatactgta
      841 aacaaaactt ccggatatgt gactgaataa aatcaaaatg ttcatctctg tag
//
