LOCUS       sequence-145954-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used for expression in E. coli and purification. N-terminal GST tag 
            and C-terminal Strep tag, aa 1-389.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pGEX6P1-human LIC1 G domain
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..33
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaatcggatc tggaagttct gttccagggg cccctgggat ccatggcggc cgtggggcga
       61 gtcggctcct tcggttcttc tccgccggga ttatcctcga cttacactgg cggccccttg
      121 ggcaacgaga tagcgtcggg caacggtggc gccgcggcag gcgacgacga ggacgggcag
      181 aacctttggt cctgcatcct cagcgaggtc tccacccgct cgcgctccaa gctccctgcg
      241 gggaagaacg tgctactgct gggtgaagat ggagctggaa aaacaagctt aataagaaaa
      301 attcagggaa tagaggagta taagaaagga agaggattgg aatatttgta cttaaatgtg
      361 catgatgaag acagggatga tcaaacaaga tgtaatgttt ggatcttaga tggagaccta
      421 tatcacaaag gcctccttaa attttcactg gatgccgtat ctctgaagga tactctagtt
      481 atgctggttg ttgacatgtc aaagccttgg actgctttgg attctttaca gaaatgggca
      541 agtgttgtta gagaacatgt tgacaaactg aaaatccctc ctgaagaaat gaaacaaatg
      601 gaacaaaagt tgattagaga cttccaagaa tatgtagagc caggagaaga cttcccggct
      661 tctccccaga gaagaaatac tgcgtcacaa gaagacaaag atgacagtgt agttttacct
      721 ctgggtgcgg atacacttac acataacttg ggcattccag tactagtagt ttgcacaaag
      781 tgtgatgcca ttagtgtatt ggagaaagaa catgactaca gagatgaaca ttttgatttt
      841 attcagtcac atatccggaa gttttgttta cagtatggtg cagcacttat ttacacttca
      901 gtaaaagaaa acaaaaatat agacttagta tataaatac
//
