LOCUS       sequence-145960-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used for expression in E. coli and purification. N-terminal GST tag 
            and C-terminal Strep tag, aa 389-523.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pGEX6P1-human LIC1 C-terminal half
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..91)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(509..532)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(559..581)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgaaacgcgc gaggcagatc gtcagtcagt cacgatgcgg ccgctcgagt cgacccggga
       61 attctcattt ttcgaactgc gggtggctcc aagaagcttc tccttccgta ggagatgtag
      121 gtgttgtggg agaaactgta actggttttc gtgtaattct gtctagttct gcatgaacat
      181 ctaagacagg cttctggcct gactttttgg tggatggtgg taaaccactg cttccacctc
      241 cagccccacc tgcagggcta ccaccactca caccagggcc tcctggagag ccagtctttt
      301 tactcaacaa actgttgaag aaatttgcca gaacgccttc acttgtagct ccagctttca
      361 tgtttggatc aatttttttt gacccagcag gaatgggtga cacgctggca acattagatg
      421 atacagatct atttggtgtt cgtggggagc ctcctgggac tcttggtgag gcatccacag
      481 gccttccagc tgcagtcatg gatcccaggg gcccctggaa cagaacttcc agatccgatt
      541 ttggaggatg gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat
      601 acttgctgga tttcaagtac ttatcaattt gtgggatagc ttcaatacgt tttttaaaac
      661 aaactaattt tgggaacgca tccaggcaca ttgggtccat gtataaaaca acatcaagag
      721 cgtcatacaa catgaagtca ggatgggtta catgatcacc atttaaatat gttttatgac
      781 ataaacgatc ttcgaacatt ttcagcattt caggtagctt gctaagaaaa tcaactttga
      841 gagtttcaaa gtctttacta tatgcaattc tcgaaacacc gtatctaata tccaaaaccg
      901 ctccttcaag cattgaaatc tctgcacgct cttttggaca accacccaac atgttgtgct
      961 tgtcagctat ataacgtatg atggccatag actgtgttaa tttaacatca ccatcaatat
     1021 a
//
