LOCUS       sequence-145975-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of CSK R107E mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Kazi JU, Vaapil M, Agarwal S, Bracco E, Pahlman S, Ronnstrand L
  TITLE     The tyrosine kinase CSK associates with FLT3 and c-Kit receptors and
            regulates downstream signaling.
  JOURNAL   Cell Signal. 2013 Sep;25(9):1852-60. doi: 
            10.1016/j.cellsig.2013.05.016. Epub 2013 May 21.
  PUBMED    23707526
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cellsig.2013.05.016"; journalName: "Cell Signal"; date: 
            "2013-09"; volume: "25"; issue: "9"; pages: "1852-60"
COMMENT     Sequence Label: pcFLAG-CSK-R107E
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tggtaccgag ctcggatcgg ccaccatggg atcctcagca
      121 atacaggccg cctggccatc cggtacagaa tgtattgcca agtacaactt ccacggcact
      181 gccgagcagg acctgccctt ctgcaaagga gacgtgctca ccattgtggc cgtcaccaag
      241 gaccccaact ggtacaaagc caaaaacaag gtgggccgtg agggcatcat cccagccaac
      301 tacgtccaga agcgggaggg cgtgaaggcg ggtaccaaac tcagcctcat gccttggttc
      361 cacggcaaga tcacacggga gcaggctgag cggcttctgt acccgccgga gacaggcctg
      421 ttcctggtgg aggagagcac caactacccc ggagactaca cgctgtgcgt gagctgcgac
      481 ggcaaggtgg agcactaccg catcatgtac catgccagca agctcagcat cgacgaggag
      541 gtgtactttg agaacctcat gcagctggtg gagcactaca cctcagacgc agatggactc
      601 tgtacgcgcc tcattaaacc aaaggtcatg gagggcacag tggcggccca ggatgagttc
      661 taccgcagcg gctgggccct gaacatgaag gagctgaagc tgctgcagac catcgggaag
      721 ggggagttcg gagacgtgat gctgggcgat taccgaggga acaaagtcgc cgtcaggtgc
      781 attaagaacg acgccactgc ccaggccttc ctggctgaag cctcagtcat gacgcaactg
      841 cggcatagca acctggtgca gctcctgggc gtgatcgtgg ag
//
