LOCUS       sequence-145980-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of CSK SH3-SH2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kazi JU, Vaapil M, Agarwal S, Bracco E, Pahlman S, Ronnstrand L
  TITLE     The tyrosine kinase CSK associates with FLT3 and c-Kit receptors and
            regulates downstream signaling.
  JOURNAL   Cell Signal. 2013 Sep;25(9):1852-60. doi: 
            10.1016/j.cellsig.2013.05.016. Epub 2013 May 21.
  PUBMED    23707526
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cellsig.2013.05.016"; journalName: "Cell Signal"; date: 
            "2013-09"; volume: "25"; issue: "9"; pages: "1852-60"
COMMENT     Sequence Label: pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             688..711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(758..775)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacttaagct tggtaccgag ctcggatcgg ccaccatggg atcctcagca
      121 atacaggccg cctggccatc cggtacagaa tgtattgcca agtacaactt ccacggcact
      181 gccgagcagg acctgccctt ctgcaaagga gacgtgctca ccattgtggc cgtcaccaag
      241 gaccccaact ggtacaaagc caaaaacaag gtgggccgtg agggcatcat cccagccaac
      301 tacgtccaga agcgggaggg cgtgaaggcg ggtaccaaac tcagcctcat gccttggttc
      361 cacggcaaga tcacacggga gcaggctgag cggcttctgt acccgccgga gacaggcctg
      421 ttcctggtgc gggagagcac caactacccc ggagactaca cgctgtgcgt gagctgcgac
      481 ggcaaggtgg agcactaccg catcatgtac catgccagca agctcagcat cgacgaggag
      541 gtgtactttg agaacctcat gcagctggtg gagcactaca cctcagacgc agatggactc
      601 tgtacgcgcc tcattaaacc aaaggtcatg gagggcacag tggcggccca ggatgagttc
      661 taccgcagcg gctgggccct gctcgaggac tacaaggatg acgatgacaa gtaagaattc
      721 gtcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
      781 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
      841 actgtccttt cctaataa
//
