LOCUS       sequence-145981-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of CSK SH3-SH2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Kazi JU, Vaapil M, Agarwal S, Bracco E, Pahlman S, Ronnstrand L
  TITLE     The tyrosine kinase CSK associates with FLT3 and c-Kit receptors and
            regulates downstream signaling.
  JOURNAL   Cell Signal. 2013 Sep;25(9):1852-60. doi: 
            10.1016/j.cellsig.2013.05.016. Epub 2013 May 21.
  PUBMED    23707526
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cellsig.2013.05.016"; journalName: "Cell Signal"; date: 
            "2013-09"; volume: "25"; issue: "9"; pages: "1852-60"
COMMENT     Sequence Label: pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..31)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      610..619
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(672..691)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(673..691)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(736..787)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(765..785)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attcttactt gtcatcgtca tccttgtagt cctcgagcag ggcccagccg ctgcggtaga
       61 actcatcctg ggccgccact gtgccctcca tgacctttgg tttaatgagg cgcgtacaga
      121 gtccatctgc gtctgaggtg tagtgctcca ccagctgcat gaggttctca aagtacacct
      181 cctcgtcgat gctgagcttg ctggcatggt acatgatgcg gtagtgctcc accttgccgt
      241 cgcagctcac gcacagcgtg tagtctccgg ggtagttggt gctctcccgc accaggaaca
      301 ggcctgtctc cggcgggtac agaagccgct cagcctgctc ccgtgtgatc ttgccgtgga
      361 accaaggcat gaggctgagt ttggtacccg ccttcacgcc ctcccgcttc tggacgtagt
      421 tggctgggat gatgccctca cggcccacct tgtttttggc tttgtaccag ttggggtcct
      481 tggtgacggc cacaatggtg agcacgtctc ctttgcagaa gggcaggtcc tgctcggcag
      541 tgccgtggaa gttgtacttg gcaatacatt ctgtaccgga tggccaggcg gcctgtattg
      601 ctgaggatcc catggtggcc gatccgagct cggtaccaag cttaagttta aacgctagcc
      661 agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc
      721 tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      781 ttgcgtcaat ggggcggagt tgttacgaca ttttggaa
//
