LOCUS       sequence-146002-        2353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo 
            P, Blanca J, Granell A, Orzaez D
  TITLE     A modular toolbox for gRNA-Cas9 genome engineering in plants based 
            on the GoldenBraid standard.
  JOURNAL   Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. 
            eCollection 2016.
  PUBMED    26839579
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2016-02-1"; volume: "12:10. doi"; pages: " 
            10.1186/s13007-016-0101-2. eCollection 2016"
COMMENT     Sequence Label: pU6-1 (GB1204)
FEATURES             Location/Qualifiers
     source          1..2353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      294..351
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(375..392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(546..1134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(626..645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1317..1411)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1432..2091)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2006..2025
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2253..2271
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2275..2318)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2321..2342
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctcgggaggt cattgtttta gttccaccac gattatattt gaaatttacg tgagtgtgag
       61 tgagacttgc ataagaaaat aaaatcttta gttgggaaaa aattcaataa tataaatggg
      121 cttgagaagg aagcgaggga taggcctttt tctaaaatag gcccatttaa gctattaaca
      181 atcttcaaaa gtaccacagc gcttaggtaa agaaagcagc tgagtttata tatggttaga
      241 gtcgaagtag tgattgtgag accactcatc gcgactagta gcggccgctg cagtccggca
      301 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
      361 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      541 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      961 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
     1321 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1381 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     1441 gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     1501 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     1561 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     1621 atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa
     1681 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     1741 tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg
     1801 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     1861 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     1921 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     1981 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     2041 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2101 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2161 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
     2221 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
     2281 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag
     2341 agcgatgagt ggt
//
