LOCUS       sequence-146003-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian DamID positive control plasmid. Expresses Cbx1-V5-Dam..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub 
            2006 Oct 12"
COMMENT     Sequence Label: pINDgw Cbx1 V5 EcoDam
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..19
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctagaaggca cagtcgaggc tgatcagcgg gtttaaactc aatggtgatg gtgatgatga
       61 ccggcttatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag cagttcgtcc
      121 acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac gacatgcaat
      181 tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat cagcactgga
      241 atatggcgct caaccagacc ttcggcgatc tccgccagat gcgcttgttg ttcaagcgta
      301 aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggtgc ataaggcgga
      361 tcgcaataga cgacggatgc atcatctgcg cgcgccatgc tatcggcgta agactcacaa
      421 tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc cgggaaatag
      481 ggttttttgt agcggccgaa cggcacgtta aactcaccgc gcagattgta acgacacagg
      541 ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa cggatcctgg
      601 cttttgttga actcttcgcg gaactgatag taaacctcgg cgcaatttgt ttcgggaaca
      661 aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat gttatagaga
      721 ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt gttgagaaac
      781 accgacccgg cacctacaaa
//
