LOCUS       sequence-146034-        3092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reb_1 with RebB(T28A, D72G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Polka JK, Silver PA
  TITLE     A Tunable Protein Piston That Breaks Membranes to Release 
            Encapsulated Cargo.
  JOURNAL   ACS Synth Biol. 2016 Feb 3.
  PUBMED    26814170
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 3."
COMMENT     Sequence Label: Reb_81 aka PAS622
FEATURES             Location/Qualifiers
     source          1..3092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     222..241
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     325..344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        325..343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    344..368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2052..2069
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2122..2140)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2136..2183
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2220..2676
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2307..2326)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2517..2538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattagagg gtgactggga tctggggcgc tctccattag gggactccgg cagtaggacg
       61 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
      121 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
      181 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
      241 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
      301 atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
      361 acaattcccc tctagagaat cagtacacac tttaaattaa ttcaaaacaa ggagctcaaa
      421 atggcgacta ctacttctac tgctgatgta ggcaacacaa ctgacatcgt aaatagccaa
      481 attactgatt cagtaacaca aactaacaca atggttcttg gttctactcc agcacaaatc
      541 atgacaaatt taatgcaaat gagcgcacaa gctaatgctt tagcaatgca aaatgctgtt
      601 gttggtcaaa aacaaacaaa tatgctatca gatgcagcaa ccactcaagg cttatctatt
      661 atgtatacgc tttcagctgc ttcagatggt caagcagtca gtactgttga caattcaact
      721 gacatggctg gactgattga tgcgcttggc gttgataaag cataagtatt aaaaatagat
      781 tatttataaa acaccgtaag ataaaatctt acgatttaaa acccagacaa agtaaggaat
      841 aaaaaatgag taatgtaaat tcacaaatta cagattcagt aacacaaaca aacactaaaa
      901 tattaggtga aatgcctgcg tttaccactg gaagcttaat gcaaatggca acacaagctg
      961 ctggcttatc tatacaaaac tctgtaacca atcagcaaca atctaatatg cttcatcagg
     1021 catcaaccac tcaaggtatg agtattctat attctgttga cacggcagct aatgctcaag
     1081 ccattggttc agtgaaccgt tctaatgaca caagccgttt aactgatgcc cttgctgtta
     1141 ttaaagccgc taaaaatggt taatgcttat ttcgatatgc taaaataatg ccaatcagat
     1201 aaattggcta aattaaagta tgataagcga aataaaatca tttaaagtag ataaaaatca
     1261 tcagagacgg ttaattgttc tcgtggggga agaaagctgg ggttaggaag tatacgttga
     1321 tttgtattat tgagtttctc ctcaacgcat aacgtattac ttcctgaccc aacgctatcg
     1381 ttttccttat tttatttttt tattttgctt tttgatatca atccatttta actagagctt
     1441 caaactatgg cagataatgc aataacaccc aatctttatg tgaatccaca agtgaccgat
     1501 tctgtcactt caaatatgat gaatgttata ggtatggcac cagctcaagc aatgggtgtg
     1561 ctgtatcaag gcgttgcttc aaccgctaat ttagctattc aaaatgctca atcaagctct
     1621 caacagttaa atcaaatcgg tcaagccgtc acttctgttg cctgtgaacg tattatgaaa
     1681 atgatgggat aatcttgtaa atggcggata tagaacattt ctcagaagat atcaatcaac
     1741 ggatgatgga tcctaaaaat aatgtcgcct ctcaaggtgc ttatgatatt gtttcaatgg
     1801 ctgccgctag cgctgtgcaa aatgaactgg ctatgcattc tggctttgaa acaacatcaa
     1861 atacggtgct agcacttgct atgcaaaaaa tggctgaagc tgccgcaatc gacaacgaac
     1921 ctcaaattaa aatatgggaa ggaatcatga aatccattag cagtaatcga gatgctgaag
     1981 tggctaatct cagtaaattt aatgagatga cacaaaactt agtgaaaaac tttccacgag
     2041 gctaactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
     2101 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     2161 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
     2221 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2281 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2341 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2401 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2461 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2521 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2581 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2641 cgcgaatttt aaacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaaatg
     2701 tgcgcggaac ccctatttgt ttattttttt ctaaaataca ttcaaaatat gtatccgctc
     2761 atgaattaat ttcttagaaa aaactcatcg agcaatcaaa atgaaaactg caattttatt
     2821 catatcagga ttatcaatac ccatattttt tgaaaaaagc cgtttctgta atgaaggaga
     2881 aaactcaccg aggcagttcc ataggatggg cagatcctgg tatcggtctg gcgattcccg
     2941 actcgtccaa catcaataca actattaatt tccccctcgt caaaataaag ggtatcagtg
     3001 aaaatcacca tgagtgacga ctgaatccgg tgaaaatggc aaaagtttta tgcatttctt
     3061 tttccagact tggtcaccag gcccggcaca ta
//
