LOCUS       sequence-146041-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of single-cysteine variant of single-stranded DNA-binding
            protein protein, EcSSB, as scaffold for ssDNA biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Fili N, Mashanov GI, Toseland CP, Batters C, Wallace MI, Yeeles JT, 
            Dillingham MS, Webb MR, Molloy JE
  TITLE     Visualizing helicases unwinding DNA at the single molecule level.
  JOURNAL   Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173.
            Epub 2010 Mar 28.
  PUBMED    20350930
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkq173"; journalName: "Nucleic Acids Res"; date: 
            "2010-07"; volume: "38"; issue: "13"; pages: "4448-57"
COMMENT     Sequence Label: pET22b_EcSSB_2
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..244
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     328..347
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    347..371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             386..408
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             999..1016
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
       61 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      121 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      181 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      241 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      301 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      361 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc
      421 cagcagaggc gtaaacaagg ttattctcgt tggtaatctg ggtcaggacc cggaagtacg
      481 ctacatgcca aatggtggcg cagttgccaa cattacgctg gctacttccg aatcctggcg
      541 tgataaagcg accggcgaga tgaaagaaca gactgaatgg caccgcgttg tgctgttcgg
      601 caaactggca gaagtggcga gcgaatatct gcgtaaaggt tctcaggttt atatcgaagg
      661 tcagctgcgt acccgtaaat gcaccgatca atccggtcag gatcgctaca ccacagaagt
      721 cgtggtgaac gttggcggca ccatgcagat gctgggtggt cgtcagggtg gtggcgctcc
      781 ggcaggtggc aatatcggtg gtggtcagcc gcagggcggt tggggtcagc ctcagcagcc
      841 gcagggtggc aatcagttca gcggcggcgc gcagtctcgc ccgcagcagt ccgctccggc
      901 agcgccgtct aacgagccgc cgatggactt tgatgatgac attccgttct gaggatccga
      961 attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag
     1021 atccggctgc taacaaagcc cgaaag
//
