LOCUS       sequence-146053-        8394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expresion of aVent1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Theunissen TW, Costa Y, Radzisheuskaya A, van Oosten AL, Lavial F, 
            Pain B, Castro LF, Silva JC
  TITLE     Reprogramming capacity of Nanog is functionally conserved in 
            vertebrates and resides in a unique homeodomain.
  JOURNAL   Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
  PUBMED    22028025
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2011-11"; volume: "138"; issue: 
            "22"; pages: "4853-65"
COMMENT     Sequence Label: pPB-CAG-aVent1-pgk-hph
FEATURES             Location/Qualifiers
     source          1..8394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..658
                     /label=chicken beta-actin promoter
     intron          659..1667
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1591..1613
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    1745..1769
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2625..2649)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2670..2686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2694..2710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2718..2724,2725..2742,2743..2748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2763..2784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2844..2861)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2850..3074
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3151..3184
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3407..3426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3408..3426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3436..3453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3436..3452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3445..3467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3594..4049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3681..3700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3891..3912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4075..4179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4180..4248,4249..5040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4398..4417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5211..5799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5700..5719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5953..5970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6087..6108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6123..6128,6129..6146,6147..6153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6161..6177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6185..6201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6220..6240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6222..6240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   6263..6297
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(6780..7805)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6780..7805)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7824..8323)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     8265..8288
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    8346..8379
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg
      721 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      781 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      841 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg
      961 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt
     1021 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct
     1141 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg
     1201 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1261 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag
     1441 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     1501 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg
     1561 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1621 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1681 tgctggttgt tgtgctgtct catcattttg gcaaagaatt cctcgacggt atcgataagc
     1741 tatcacaagt ttgtacaaaa aagcaggctt caccatgact gcctgcaagc tccccttcgc
     1801 tctctccgtg gaaaacttga tcggcgctcg aactgacctt gttcctgaca tcagagcttc
     1861 cagccccgaa tgtcttccac cgaagaagcg tcacctcctg ttcaccgcct cgccgggccc
     1921 ggacgcaaac actcggaccg ccgctcaagt cgtgccgccc gccgctaacg ctctgcggag
     1981 tgacggagaa acgtctgacg aggaggagga gatcgacgtc gtttccattg gggacgcagc
     2041 ctcacctgct tccagcacga gttcagaccg ctccagccca gcaccttcct gcgacgacag
     2101 cccgtctccc tccccggcag gccgcaaggc acgcacggcc ttcaccaccg agcaggtcat
     2161 ggccctggag gagcgtttcc gcctgcagaa gtacctgtcc gccgccgacc gggaaactct
     2221 ggccaaggcc acgggactga ccgacgagca ggtcaagacg tggttccaga accgccgtat
     2281 gaagctgaag cgccagcagc aagacttcgc caccttcccg ctgcacgcct ccgtcaccgt
     2341 gcccgggatg cccggctact ccccacagcc caacccctgg tacgactcgg cgcacttcgt
     2401 cccgtccctc ggcaccgccg tctgtggtct gcggcctcac atgctgcctc agcccgccaa
     2461 caccgtgacc accagccgtg ggaacagata ctcaccgtac ccgtctgccc gcgtgtctcc
     2521 gtccggccgc agccaccccc agatgaccaa catgtacatg tctccgtacc agaacaacgt
     2581 ggcaggggcg cactacaaca acgtacccag catgaacttc taagacccag ctttcttgta
     2641 caaagtggtg atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2701 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2761 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2821 aacctgtcgt gccagcggat cagcctcgac tgtgccttct agttgccagc catctgttgt
     2881 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     2941 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     3001 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     3061 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgagat ccactagttc
     3121 tagcctcgag gctagagcgg ccgcgtcgag ataacttcgt ataatgtatg ctatacgaag
     3181 ttatgttttg ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat
     3241 aaacataaat aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa
     3301 cttaatatct attcaaatta ataaataaac gtcaatttta cgcatgatta tctttaacgt
     3361 acgtcacaat atgattatct ttctagggtt aaagagctcc aattcgccct atagtgagtc
     3421 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3481 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3541 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3721 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3781 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3841 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3901 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3961 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4021 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4081 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4141 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4201 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4261 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4321 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4381 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4441 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4501 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4561 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4621 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4681 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4741 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4801 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4861 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4921 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4981 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5041 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5101 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5341 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6001 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6061 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6181 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6241 gaacaaaagc tgggtacctt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
     6301 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
     6361 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
     6421 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
     6481 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
     6541 attatatata tattttcttg ttatagatat ccaactatga aacattatca taaagcaata
     6601 gtaaagagcc ttcagtaaag agcaggcatt tatctaatcc caccccaccc ccacccccgt
     6661 agctccaatc cttccattca aaatgtaggt actctgttct cacccttctt aacaaagtat
     6721 gacaggaaaa aacttccatg tggacatctt tattgtttaa tagatcatca atttctcgac
     6781 tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct
     6841 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc
     6901 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa
     6961 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg
     7021 agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc
     7081 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg
     7141 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg
     7201 ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc
     7261 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc
     7321 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga
     7381 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg
     7441 atcgcatcca tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg
     7501 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat
     7561 tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca
     7621 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct
     7681 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag
     7741 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt
     7801 ttcatggtgg cggctggatc ggtcgaaagg cccggagatg aggaagagga gaacagcgcg
     7861 gcagacgtgc gcttttgaag cgtgcgagaa tgccgggcct ccggaggacc ttcgggcgcc
     7921 cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg
     7981 agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt
     8041 ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg
     8101 tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt
     8161 agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt
     8221 ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgccagcgg ggctgctaaa
     8281 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttcatcgatg
     8341 atatcataac ttcgtataat gtatgctata cgaagttatg cggccctcga ggtc
//
