LOCUS       sequence-146066-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA expression vector for mouse Dedd gene targeting intron 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     pUC57-sgDedd-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC57-sgDedd-2
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..191
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     183..200
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     184..200
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        247..265
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        287..362
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(420..436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(420..436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(433..455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    444..460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(468..474,475..492,493..498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    513..534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(652..669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
       61 gcgcaactgt ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      121 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      181 gttgtaaaac gacggccagt gaattcgagc tcggtacctc gcgaatgcat ctagatatcg
      241 gatccctaat acgactcact ataggagtgc ctcatgtaaa ggtcaggttt tagagctaga
      301 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      361 gcttttttta aagggcccgt cgactgcaga ggcctgcatg caagcttggc gtaatcatgg
      421 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      481 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      541 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      601 ggccaacgcg cggggagagg cggtttgcgt attgggcgcg gccgccgctt cctcgctcac
      661 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      721 aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag
      781 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag ctccgccccc
      841 ctgacgagca tcacaaaaat cgacgctcaa gtcagaagtg gcgaaacccg ac
//
